Chemical and pharmacological characterization of aqueous and ethanolic extracts of Cyclamen hederifolium Ait. (Primulaceae) tuber

Background/Aim. Cyclamen hederifolium (C. hederifolium) Ait. belongs to the family Primulaceae, which includes 23 species of cyclamen, naturally distributed in Central and Southern Europe, Western Asia some parts North Africa. This plant is considered highly poisonous not suitable for human use. However, tuber extracts have been used traditional medicine homeopathy. The aim this study was investigate C. growing Serbia its metal content biological activities (anti-oxidant, antibacterial, antifungal cytotoxic activity). Methods. Content metals determined by atomic absorption spectrophotometric method. We several different assays assessment antioxidant activity both aqueous ethanol hederifolium: 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) ferric-reducing power (FRAP) total reducing assay (TRP) cupric capacity (CUPRAC) assay. disk diffusion sensitivity laboratory bacterial fungal control strains against investigated extracts. Aqueous were examined on 4 tumoral cell lines vitro MTT bioassay. Results. presence Mn, Ca, Mg, Fe, Zn, K, Cu confirmed extract, as well whole tubers soil, while Cr, Ni, Pb Cd detected. Both extract showed activity, that positively correlated phenols flavonoids it. slightly superior these terms than one. None tested antimicrobial activity. cytotoxicity four cancer 4T1, HCT116, CT26, LLC1, concentration range 15,625?2,000 ?g/mL. Ethanol stronger ex-tract. Conclusion. Seven identified tubers, soil. exhibited